Journal: International Journal of Molecular Sciences

Article Title: Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

doi: 10.3390/ijms232113184

Figure Lengend Snippet: The protein expression of NK1R ( A ) and NK2R ( B ) in the porcine myometrium from the control (CON), saline (SAL) and E. coli groups, estimated by Western blot analysis. Blots with representative bands for each group are shown in . Protein levels of studied receptors are expressed as the mean ± SEM of ratios of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). ** p < 0.01, *** p < 0.001 compared between groups for the same type of receptor.

Article Snippet: Afterwards, membranes were incubated with rabbit polyclonal antibodies against NK1R (Novus Biologicals, Littleton, CO, USA, cat. no. NB 300-119, working dilution 1:500) and NK2R (LifeSpan BioSciences, Inc., Seattle, 161 WA, USA, cat. no. LS-C177106, working dilution 1:500) at 4 °C, for 18 h. In the next step, the membranes were washed in TBS-T buffer and then incubated (at 21 °C, for 1 h) with biotinylated goat anti-rabbit IgG (Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA, cat. no. PK-6101, working dilution 1:3000).

Techniques: Expressing, Control, Saline, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

doi: 10.3390/ijms232113184

Figure Lengend Snippet: Representative pictures of NK1R ( A – C ) and NK2R ( E – G ) in the myometrium of gilts from the control (CON), saline (SAL) and E. coli ( E. coli ) groups. Positive immunoreaction to NK1R is visible in myometrial muscular cells (MCM) and arteries ( A ) of the control ( A ), saline-( B ) and pathogens ( C )-injected uteri. Expression of NK2R display MCM and A in the control ( E ), saline-injected ( F ) and inflamed ( G ) uteri. Negative control (NC) for NK1R ( D ) and NK2R ( H ) was obtained following the use of rabbit normal IgG instead of primary antibodies.

Article Snippet: Afterwards, membranes were incubated with rabbit polyclonal antibodies against NK1R (Novus Biologicals, Littleton, CO, USA, cat. no. NB 300-119, working dilution 1:500) and NK2R (LifeSpan BioSciences, Inc., Seattle, 161 WA, USA, cat. no. LS-C177106, working dilution 1:500) at 4 °C, for 18 h. In the next step, the membranes were washed in TBS-T buffer and then incubated (at 21 °C, for 1 h) with biotinylated goat anti-rabbit IgG (Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA, cat. no. PK-6101, working dilution 1:3000).

Techniques: Control, Saline, Injection, Expressing, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

doi: 10.3390/ijms232113184

Figure Lengend Snippet: The influence of substance P (SP) on the contractile amplitude ( A , C ) and frequency ( B , D ) in the myometrium ( A , B ) and endometrium/myometrium ( C , D ) strips of gilts from the CON (grey bars), SAL (hatched bars) and E. coli (black bars) groups after using NK1R antagonist. Results were calculated for five gilts in each group. The effects of antagonist and individual SP doses are presented as percentages (mean ± SEM) of the baseline (pretreatment period) contractile amplitude and frequency, taken as 100% (horizontal lines). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the basal value in each group; AAA p < 0.001 compared between the CON and E. coli groups for the same treatment; B p < 0.05, BB p < 0.01, BBB p < 0.001 compared between the SAL and E. coli groups for the same treatment.

Article Snippet: Afterwards, membranes were incubated with rabbit polyclonal antibodies against NK1R (Novus Biologicals, Littleton, CO, USA, cat. no. NB 300-119, working dilution 1:500) and NK2R (LifeSpan BioSciences, Inc., Seattle, 161 WA, USA, cat. no. LS-C177106, working dilution 1:500) at 4 °C, for 18 h. In the next step, the membranes were washed in TBS-T buffer and then incubated (at 21 °C, for 1 h) with biotinylated goat anti-rabbit IgG (Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA, cat. no. PK-6101, working dilution 1:3000).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

doi: 10.3390/ijms232113184

Figure Lengend Snippet: The graphic shows the procedure for measuring contractility of uterine strips. Ach-acetylcholine; SP-substance P; NKA-neurokinin A; NK1R-neurokinin receptor subtype 1; NK2R-neurokinin receptor subtype 2; NK1R anta.-neurokinin receptor subtype 1 antagonist; NK2R anta.-neurokinin receptor subtype 2 antagonist. Concentrations of the used substances are given in moles.

Article Snippet: Afterwards, membranes were incubated with rabbit polyclonal antibodies against NK1R (Novus Biologicals, Littleton, CO, USA, cat. no. NB 300-119, working dilution 1:500) and NK2R (LifeSpan BioSciences, Inc., Seattle, 161 WA, USA, cat. no. LS-C177106, working dilution 1:500) at 4 °C, for 18 h. In the next step, the membranes were washed in TBS-T buffer and then incubated (at 21 °C, for 1 h) with biotinylated goat anti-rabbit IgG (Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA, cat. no. PK-6101, working dilution 1:3000).

Techniques: